Composite

Part:BBa_K593011

Designed by: Ceren Seref   Group: iGEM11_METU-Ankara   (2011-09-21)


ROSE regulated GFP generator

This part is composed of T7 promoter, ROSE RNA thermometer and GFP. When temperature is elevated to 42C, translation is initiated and GFP expression occurs. This part is designed to measure the temperature depended efficiency of ROSE RNA thermometer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


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Parameters
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